A step forward in the genome characterization of the sugarcane borer, Diatraea saccharalis: karyotype analysis, sex chromosome systém and repetitive DNAs through a cytogenomic approach
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00562331" target="_blank" >RIV/60077344:_____/22:00562331 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/22:43905326
Result on the web
<a href="https://link.springer.com/article/10.1007/s00412-022-00781-4" target="_blank" >https://link.springer.com/article/10.1007/s00412-022-00781-4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00412-022-00781-4" target="_blank" >10.1007/s00412-022-00781-4</a>
Alternative languages
Result language
angličtina
Original language name
A step forward in the genome characterization of the sugarcane borer, Diatraea saccharalis: karyotype analysis, sex chromosome systém and repetitive DNAs through a cytogenomic approach
Original language description
Moths of the family Crambidae include a number of pests that cause economic losses to agricultural crops. Despite their economic importance, little is known about their genome architecture and chromosome evolution. Here, we characterized the chromosomes and repetitive DNA of the sugarcane borer Diatraea saccharalis using a combination of low-pass genome sequencing, bioinformatics, and cytogenetic methods, focusing on the sex chromosomes. Diploid chromosome numbers differed between the sexes, i.e., 2n = 33 in females and 2n = 34 in males. This difference was caused by the occurrence of a WZ 1 Z 2 trivalent in female meiosis, indicating a multiple sex-chromosome system WZ 1 Z 2 /Z 1 Z 1 Z 2 Z 2 . A strong interstitial telomeric signal was observed on the W chromosome, indicating a fusion of the ancestral W chromosome with an autosome. Among repetitive DNAs, transposable elements (TEs) accounted for 39.18% (males) to 41.35% (females), while satDNAs accounted for only 0.214% (males) and 0.215% (females) of the genome. FISH mapping revealed different chromosomal organization of satDNAs, such as single localized clusters, spread repeats, and non-clustered repeats. Two TEs mapped by FISH were scattered. Although we found a slight enrichment of some satDNAs in the female genome, they were not differentially enriched on the W chromosome. However, we found enriched FISH signals for TEs on the W chromosome, suggesting their involvement in W chromosome degeneration and differentiation. These data shed light on karyotype and repetitive DNA dynamics due to multiple chromosome fusions in D. saccharalis, contribute to the understanding of genome structure in Lepidoptera and are important for future genomic studies.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10603 - Genetics and heredity (medical genetics to be 3)
Result continuities
Project
<a href="/en/project/GA20-13784S" target="_blank" >GA20-13784S: Chromosomal and molecular mechanisms of sex determination in Lepidoptera</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chromosoma
ISSN
0009-5915
e-ISSN
1432-0886
Volume of the periodical
131
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
15
Pages from-to
253-267
UT code for WoS article
000865893800002
EID of the result in the Scopus database
2-s2.0-85139611804