Codling moth cytogenetics: karyotype, chromosomal location of rDNA, and molecular differentiation of sex chromosomes
Result description
We performed a detailed karyotype analysis in the codling moth, Cydia pomonella, the key pest of pome fruit in the temperate regions of the world. The karyotype consisted of 2n = 56 chromosomes. In pachytene nuclei, a curious NOR (nucleolar organizer region) bivalent was observed. It carried 2 nucleoli, each associated with one end of the bivalent as confirmed by FISH with an 18S ribosomal DNA, which detected 2 clusters of rRNA genes. Pachytene oocytes showed a sex chromosome bivalent (WZ). Molecular differentiation of the sex chromosomes was studied using genomic in situ hybridization (GISH) and comparative genomic hybridization (CGH). GISH detected the W chromosome by strong binding of the female-derived DNA probe. With CGH, both the female-derived probe and male-derived probe evenly bound to the W chromosome. This suggested that the W chromosome is predominantly composed of repetitive DNA sequences occurring scattered in other chromosomes but accumulated in the W chromosome.
Keywords
The result's identifiers
Result code in IS VaVaI
Alternative codes found
RIV/60076658:12310/05:00008704
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Codling moth cytogenetics: karyotype, chromosomal location of rDNA, and molecular differentiation of sex chromosomes
Original language description
We performed a detailed karyotype analysis in the codling moth, Cydia pomonella, the key pest of pome fruit in the temperate regions of the world. The karyotype consisted of 2n = 56 chromosomes. In pachytene nuclei, a curious NOR (nucleolar organizer region) bivalent was observed. It carried 2 nucleoli, each associated with one end of the bivalent as confirmed by FISH with an 18S ribosomal DNA, which detected 2 clusters of rRNA genes. Pachytene oocytes showed a sex chromosome bivalent (WZ). Molecular differentiation of the sex chromosomes was studied using genomic in situ hybridization (GISH) and comparative genomic hybridization (CGH). GISH detected the W chromosome by strong binding of the female-derived DNA probe. With CGH, both the female-derived probe and male-derived probe evenly bound to the W chromosome. This suggested that the W chromosome is predominantly composed of repetitive DNA sequences occurring scattered in other chromosomes but accumulated in the W chromosome.
Czech name
Cytogenetika obaleče jablečného: karyotyp, chromosomální lokalizace rDNA a molekulární diferenciace pohlavních chromosomů
Czech description
Provedli jsme detailní analýzu karyotypu obaleče jablečného, Cydia pomonella, klíčového škůdce jádrového ovoce v oblastech mírného pásu celého světa. Karyotyp byl tvořen 2n = 56 chromosomy. V pachytenních jádrech jsme pozorovali kuriózni bivalent, nesoucí 2 organizátory jadérek (NOR), každý na opačném konci. Toto potvrdila i metoda FISH se sondou pro 18S ribosomální DNA, která detekovala 2 klastry rRNA genů. Pachytenní oocyty vykazovaly pár pohlavních chromosomů WZ. Pro studium jejich molekulární diferenciace jsme použili genomovou in situ hybridizaci (GISH) a komparativní genomovou hybridizaci (CGH). GISH detekovala chromosom W silnou vazbou se samičí genomovou DNA sondou. Při CGH chromosom W hybridizoval stejnoměrně se značenou samičí i samčí genomovou sondou. Z toho lze vyvodit, že chromosom W je tvořen převážně repetitivními sekvencemi DNA rozptýlenými po celém genomu, avšak akumulovanými na chromosomu W.
Classification
Type
Jx - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
IAA6007307: Role of the sex chromosome W in sex determination of Lepidoptera
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Genome
ISSN
0831-2796
e-ISSN
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Volume of the periodical
48
Issue of the periodical within the volume
-
Country of publishing house
CA - CANADA
Number of pages
10
Pages from-to
1083-1092
UT code for WoS article
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EID of the result in the Scopus database
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Basic information
Result type
Jx - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP
EB - Genetics and molecular biology
Year of implementation
2005