Genetics tools for corpora allata specific gene expression in Aedes aegypti mosquitoes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00565394" target="_blank" >RIV/60077344:_____/22:00565394 - isvavai.cz</a>
Result on the web
<a href="https://www.nature.com/articles/s41598-022-25009-4" target="_blank" >https://www.nature.com/articles/s41598-022-25009-4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-022-25009-4" target="_blank" >10.1038/s41598-022-25009-4</a>
Alternative languages
Result language
angličtina
Original language name
Genetics tools for corpora allata specific gene expression in Aedes aegypti mosquitoes
Original language description
Juvenile hormone (JH) is synthesized by the corpora allata (CA) and controls development and reproduction in insects. Therefore, achieving tissue-specific expression of transgenes in the CA would be beneficial for mosquito research and control. Different CA promoters have been used to drive transgene expression in Drosophila, but mosquito CA-specific promoters have not been identified. Using the CRISPR/Cas9 system, we integrated transgenes encoding the reporter green fluorescent protein (GFP) close to the transcription start site of juvenile hormone acid methyl transferase (JHAMT), a locus encoding a JH biosynthetic enzyme, specifically and highly expressed in the CA of Aedes aegypti mosquitoes. Transgenic individuals showed specific GFP expression in the CA but failed to reproduce the full pattern of jhamt spatiotemporal expression. In addition, we created GeneSwitch driver and responder mosquito lines expressing an inducible fluorescent marker, enabling the temporal regulation of the transgene via the presence or absence of an inducer drug. The use of the GeneSwitch system has not previously been reported in mosquitoes and provides a new inducible binary system that can control transgene expression in Aedes aegypti.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GA22-21244S" target="_blank" >GA22-21244S: Deciphering juvenile hormone (JH) dependent regulation of development and reproduction in mosquitoes using JH null mutants</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
2045-2322
Volume of the periodical
12
Issue of the periodical within the volume
1
Country of publishing house
DE - GERMANY
Number of pages
9
Pages from-to
20426
UT code for WoS article
000889945500088
EID of the result in the Scopus database
2-s2.0-85142868924