Differential gene expression and microRNA profile in <i>corpora allata-corpora cardiaca</i> of <i>Aedes aegypti</i> mosquitoes with weak juvenile hormone signalling

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00582766" target="_blank" >RIV/60077344:_____/24:00582766 - isvavai.cz</a>

Result on the web

<a href="https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-024-10007-9" target="_blank" >https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-024-10007-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12864-024-10007-9" target="_blank" >10.1186/s12864-024-10007-9</a>

Result language

angličtina

Original language name

Differential gene expression and microRNA profile in <i>corpora allata-corpora cardiaca</i> of <i>Aedes aegypti</i> mosquitoes with weak juvenile hormone signalling

Original language description

The corpora allata-corpora cardiaca (CA-CC) is an endocrine gland complex that regulates mosquito development and reproduction through the synthesis of juvenile hormone (JH). Epoxidase (Epox) is a key enzyme in the production of JH. We recently utilized CRISPR/Cas9 to establish an epoxidase-deficient (epox(-/-)) Aedes aegypti line. The CA from epox(-/-) mutants do not synthesize epoxidated JH III but methyl farneosate (MF), a weak agonist of the JH receptor, and therefore have reduced JH signalling. Illumina sequencing was used to examine the differences in gene expression between the CA-CC from wild type (WT) and epox(-/-) adult female mosquitoes. From 18,034 identified genes, 317 were significantly differentially expressed. These genes are involved in many biological processes, including the regulation of cell proliferation and apoptosis, energy metabolism, and nutritional uptake. In addition, the same CA-CC samples were also used to examine the microRNA (miRNA) profiles of epox(-/- )and WT mosquitoes. A total of 197 miRNAs were detected, 24 of which were differentially regulated in epox(-/-) mutants. miRNA binding sites for these particular miRNAs were identified using an in silico approach, they target a total of 101 differentially expressed genes. Our results suggest that a lack of epoxidase, besides affecting JH synthesis, results in the diminishing of JH signalling that have significant effects on Ae. aegypti CA-CC transcriptome profiles, as well as its miRNA repertoire.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/GA22-21244S" target="_blank" >GA22-21244S: Deciphering juvenile hormone (JH) dependent regulation of development and reproduction in mosquitoes using JH null mutants</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BMC Genomics

ISSN

1471-2164

e-ISSN

1471-2164

Volume of the periodical

25

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

113

UT code for WoS article

001151282500001

EID of the result in the Scopus database

2-s2.0-85183046576