Ovarian transcriptome analyses indicate that weak juvenile hormone signaling underlies the molecular basis of oogenesis deficiencies in mosquitoes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00636637" target="_blank" >RIV/60077344:_____/25:00636637 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/25:43909967
Result on the web
<a href="https://doi.org/10.1186/s12915-025-02266-z" target="_blank" >https://doi.org/10.1186/s12915-025-02266-z</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s12915-025-02266-z" target="_blank" >10.1186/s12915-025-02266-z</a>
Alternative languages
Result language
angličtina
Original language name
Ovarian transcriptome analyses indicate that weak juvenile hormone signaling underlies the molecular basis of oogenesis deficiencies in mosquitoes
Original language description
Background Juvenile hormone (JH) is synthesized by the corpora allata (CA) and controls development and reproduction in insects. We recently used CRISPR/Cas9 to establish a line lacking the enzyme that catalyzes the final step of JH biosynthesis in mosquitoes, a P450 epoxidase. The CA of the epox-/- mutants do not synthesize epoxidized JH III but methyl farneosate (MF), a weak agonist of the JH receptor. Female epox-/- mosquitoes have reduced JH signaling and show a substantial loss of reproductive fitness. To understand the molecular basis of this loss of fitness, we constructed ovarian mRNA libraries of Ae. aegypti of the Orlando strain wild-type (WT) and epoxidase null mutants (epox-/-) and investigated differential expression of reproductive genes.ResultsWe performed triplicate RNA-seq analyses of female WT and epox-/- ovaries dissected at four critical stages of oogenesis: Ovaries from newly eclosed females (0h), sugar-fed females at 4 days post-eclosion (4d SF), females 16h (16h BF), and 48 h after a blood meal (48h BF). Silencing of epoxidase resulted in a drastic change in the expression of thousands of genes.ConclusionsOur results suggest that epoxidase deficiency leads to a reduction in JH signaling that has significant effects on Ae. aegypti ovarian transcriptome profiles. Ecdysteroid titers are dysregulated in the mutants, leading to a significant delay in the expression of vitelline membrane genes and other transcripts. We discovered changes in the expression of 230 long non-coding RNAs (lncRNAs) that may play an important role in the regulation of ovarian genes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
BMC Biology
ISSN
1741-7007
e-ISSN
1741-7007
Volume of the periodical
23
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
16
Pages from-to
160
UT code for WoS article
001503873700003
EID of the result in the Scopus database
2-s2.0-105007539250