Comparison of molecular diagnostic approaches for the detection and differentiation of the intestinal protist Blastocystis sp. in humans

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00569079" target="_blank" >RIV/60077344:_____/22:00569079 - isvavai.cz</a>
Alternative codes found
RIV/68081766:_____/22:00558113 RIV/60076658:12310/22:43905006 RIV/00064203:_____/22:10444203 RIV/00216208:11130/22:10444203
Result on the web
<a href="https://www.parasite-journal.org/articles/parasite/full_html/2022/01/parasite220019/parasite220019.html" target="_blank" >https://www.parasite-journal.org/articles/parasite/full_html/2022/01/parasite220019/parasite220019.html</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1051/parasite/2022029" target="_blank" >10.1051/parasite/2022029</a>

Result language
angličtina
Original language name
Comparison of molecular diagnostic approaches for the detection and differentiation of the intestinal protist Blastocystis sp. in humans
Original language description
Blastocystis is the most commonly found intestinal protist in the world. Accurate detection and differentiation of Blastocystis including its subtypes (arguably species) are essential to understand its epidemiology and role in human health. We compared (i) the sensitivity of conventional PCR (cPCR) and qPCR in a set of 288 DNA samples obtained from stool samples of gut-healthy individuals, and (ii) subtype diversity as detected by next-generation sequencing (NGS) versus Sanger sequencing. Real-time PCR resulted in more positive samples than cPCR, revealing high fecal load of Blastocystis based on the quantification curve in most samples. In subtype detection, NGS was largely in agreement with Sanger sequencing but showed higher sensitivity for mixed subtype colonization within one host. This fact together with use of the combination of qPCR and NGS and obtaining information on the fecal protist load will be beneficial for epidemiological and surveillance studies.
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30310 - Parasitology

Project
<a href="/en/project/GA22-04837S" target="_blank" >GA22-04837S: Associations between protists and bacteriome in the gut ecosystem of household dogs and their owners across a transition to modern lifestyle</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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