A cross-sectional survey of Blastocystis sp. and Dientamoeba fragilis in non-human primates and their caregivers in Czech zoos
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00604830" target="_blank" >RIV/60077344:_____/24:00604830 - isvavai.cz</a>
Alternative codes found
RIV/68081766:_____/24:00588098 RIV/60076658:12310/24:43908785
Result on the web
<a href="https://doi.org/10.1016/j.onehlt.2024.100862" target="_blank" >https://doi.org/10.1016/j.onehlt.2024.100862</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.onehlt.2024.100862" target="_blank" >10.1016/j.onehlt.2024.100862</a>
Alternative languages
Result language
angličtina
Original language name
A cross-sectional survey of Blastocystis sp. and Dientamoeba fragilis in non-human primates and their caregivers in Czech zoos
Original language description
Intestinal protists in the gut microbiome are increasingly studied, but their basic epidemiology is not well understood. We explored the prevalence, genetic diversity, and potential zoonotic transmission of two protists colonizing the large intestine Blastocystis sp. and Dientamoeba fragilis in 37 species of non-human primates (NHPs) and their caregivers in six zoos in the Czech Republic. We analyzed 179 fecal samples (159 from NHPs, 20 from humans) by qPCR. Blastocystis sp. was detected in 54.7% (98/179) of samples, in 24 NHP species and in 57.2% of NHP samples (prevalence ranged between 36 and 80%), and in 35% of human samples (prevalence ranged between 0 and 67%). Using next generation amplicon sequencing, nine Blastocystis subtypes (ST1-ST5, ST7, ST8, and two novel subtypes) were identified. The two new Blastocystis subtypes (named ST47 and ST48) were described using Nanopore sequencing to produce full-length reference sequences of the small subunit ribosomal RNA gene. Some subtypes were shared between NHPs and their caregivers, suggesting potential zoonotic transmission. Mixed subtype colonization was frequently observed, with 52% of sequenced samples containing two or more subtypes. Dientamoeba was found only in NHPs with a prevalence of 6%. This study emphasizes the critical role of molecular diagnostics in epidemiological and transmission studies of these protists and calls for further research to better understand their impact on public health.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30310 - Parasitology
Result continuities
Project
<a href="/en/project/GA22-04837S" target="_blank" >GA22-04837S: Associations between protists and bacteriome in the gut ecosystem of household dogs and their owners across a transition to modern lifestyle</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
One Health
ISSN
2352-7714
e-ISSN
2352-7714
Volume of the periodical
19
Issue of the periodical within the volume
DEC
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
12
Pages from-to
100862
UT code for WoS article
001280688500001
EID of the result in the Scopus database
2-s2.0-85199348613