Deviations in the Z:A ratio disrupt sexual development in the eri silkmoth, Samia cynthia ricini

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F23%3A00571014" target="_blank" >RIV/60077344:_____/23:00571014 - isvavai.cz</a>

Result on the web

<a href="https://academic.oup.com/genetics/article-pdf/224/1/iyad023/50190185/iyad023.pdf" target="_blank" >https://academic.oup.com/genetics/article-pdf/224/1/iyad023/50190185/iyad023.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/genetics/iyad023" target="_blank" >10.1093/genetics/iyad023</a>

Result language

angličtina

Original language name

Deviations in the Z:A ratio disrupt sexual development in the eri silkmoth, Samia cynthia ricini

Original language description

Moths and butterflies (Lepidoptera) have sex chromosome systems with female heterogamety, and 2 models, W-dominance and Z-counting, have been proposed to determine sex. The W-dominant mechanism is well known in Bombyx mori. However, little is known about the Z-counting mechanism in Z0/ZZ species. We investigated whether ploidy changes affect sexual development and gene expression in the eri silkmoth, Samia cynthia ricini (2n = 27♀/28♂, Z0♀/ZZ♂). Tetraploid males (4n = 56, ZZZZ) and females (4n = 54, ZZ) were induced by heat and cold shock, and then, triploid embryos were produced by crosses between diploids and tetraploids. Two karyotypes (3n = 42, ZZZ and 3n = 41, ZZ) were identified in triploid embryos. Triploid embryos with 3 Z chromosomes showed male-specific splicing of the S. cynthia doublesex (Scdsx) gene, whereas 2-Z triploid embryos showed both male- and female-specific splicing. From larva to adult, 3-Z triploids showed a normal male phenotype, except for defects in spermatogenesis. However, abnormal gonads were observed in 2-Z triploids, which showed both male- and female-specific Scdsx transcripts not only in the gonads but also in somatic tissues. Two-Z triploids were thus obviously intersexes, suggesting that sexual development in S. c. ricini depends on the Z:A ratio and not only on the Z number. Moreover, mRNA-seq analyses in embryos showed that relative levels of gene expression are similar between samples with different doses of Z chromosomes and autosome sets. Our results provide the first evidence that ploidy changes disrupt sexual development but have no effect on the general mode of dosage compensation in Lepidoptera.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10603 - Genetics and heredity (medical genetics to be 3)

Project

<a href="/en/project/GA20-13784S" target="_blank" >GA20-13784S: Chromosomal and molecular mechanisms of sex determination in Lepidoptera</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Genetics

ISSN

0016-6731

e-ISSN

1943-2631

Volume of the periodical

224

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

iyad023

UT code for WoS article

000973578500001

EID of the result in the Scopus database

2-s2.0-85159553004