Distant genetic variants of Anaplasma phagocytophilum from Ixodes ricinus attached to people
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F23%3A00571513" target="_blank" >RIV/60077344:_____/23:00571513 - isvavai.cz</a>
Alternative codes found
RIV/60460709:41210/23:95179 RIV/00216224:14310/23:00132145 RIV/62156489:43210/23:43923149 RIV/00216208:11140/23:10457907 RIV/62157124:16810/23:43880560
Result on the web
<a href="https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-023-05654-y" target="_blank" >https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-023-05654-y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s13071-023-05654-y" target="_blank" >10.1186/s13071-023-05654-y</a>
Alternative languages
Result language
angličtina
Original language name
Distant genetic variants of Anaplasma phagocytophilum from Ixodes ricinus attached to people
Original language description
Background Although the tick-borne pathogen Anaplasma phagocytophilum is currently described as a single species, studies using genetic markers can distinguish groups of variants associated with different hosts, pathogenicity, zoonotic potential and biotic and geographic niches. The objective of our study was to investigate the genetic diversity of A. phagocytophilum and Ixodes ricinus ticks attached to people.Methods In collaboration with a commercial diagnostic company, a total of 52 DNA samples were obtained from ticks that tested positive for A. phagocytophilum by quantitative PCR. The genetic profile of each sample was determined using the groEL and ankA genes. Identification of the tick species was confirmed by partial sequencing of the COI subunit and a portion of the TROSPA gene.Results All 52 ticks were identified as I. ricinus. Two protocols of nested PCR amplifying 1293- and 407-bp fragments of groEL of A. phagocytophilum yielded amplicons of the expected size for all 52 samples. Among all sequences, we identified 10 unique genetic variants of groEL belonging to ecotype I and ecotype II. The analysis targeting ankA was successful in 46 of 52 ticks. Among all sequences, we identified 21 unique genetic variants phylogenetically belonging to three clusters.Conclusions Our results indicate that ticks attached to people harbor distant genetic variants of A. phagocytophilum, some of which are not recognized as zoonotic. Further studies are needed to determine the risk of human infection by genetic variants other than those designated as zoonotic.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10607 - Virology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Parasites & Vectors
ISSN
1756-3305
e-ISSN
1756-3305
Volume of the periodical
16
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
80
UT code for WoS article
000941325000003
EID of the result in the Scopus database
2-s2.0-85149409219