Conformational transition of the Ixodes ricinus salivary serpin Iripin-4

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F23%3A00572377" target="_blank" >RIV/60077344:_____/23:00572377 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/23:43906526
Result on the web
<a href="https://scripts.iucr.org/cgi-bin/paper?S2059798323002322" target="_blank" >https://scripts.iucr.org/cgi-bin/paper?S2059798323002322</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1107/S2059798323002322" target="_blank" >10.1107/S2059798323002322</a>

Result language
angličtina
Original language name
Conformational transition of the Ixodes ricinus salivary serpin Iripin-4
Original language description
Iripin-4, one of the many salivary serpins from Ixodes ricinus ticks with an as-yet unexplained function, crystallized in two different structural conformations, namely the native partially relaxed state and the cleaved serpin. The native structure was solved at a resolution of 2.3 angstrom and the structure of the cleaved conformation was solved at 2.0 angstrom resolution. Furthermore, structural changes were observed when the reactive-centre loop transitioned from the native conformation to the cleaved conformation. In addition to this finding, it was confirmed that Glu341 represents a primary substrate-recognition site for the inhibitory mechanism. The presence of glutamate instead of the typical arginine in the P1 recognition site of all structurally characterized I. ricinus serpins (PDB entries 7b2t, 7pmu and 7ahp), except for the tyrosine in the P1 site of Iripin-2 (formerly IRS-2, PDB entry 3nda), would explain the absence of inhibition of the tested proteases that cleave their substrate after arginine. Further research on Iripin-4 should focus on functional analysis of this interesting serpin.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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