Masculinizer gene controls male sex determination in the codling moth, Cydia pomonella

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F23%3A00574095" target="_blank" >RIV/60077344:_____/23:00574095 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/23:43907234

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0965174823000851/pdfft?md5=70a9a32dc6a58aacc943d40ee7d3fb8b&pid=1-s2.0-S0965174823000851-main.pdf" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0965174823000851/pdfft?md5=70a9a32dc6a58aacc943d40ee7d3fb8b&pid=1-s2.0-S0965174823000851-main.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ibmb.2023.103991" target="_blank" >10.1016/j.ibmb.2023.103991</a>

Result language

angličtina

Original language name

Masculinizer gene controls male sex determination in the codling moth, Cydia pomonella

Original language description

The molecular mechanisms of sex determination in moths and butterflies (Lepidoptera) with female heterogamety (WZ/ZZ) are poorly understood, except in the silkworm Bombyx mori. However, the Masculinizer (Masc) gene that controls male development and dosage compensation in B. mori, appears to be conserved in Lepidoptera, as its masculinizing function was recently confirmed in several moth species. In this work, we investigated the role of the Masc gene in sex determination of the codling moth Cydia pomonella (Tortricidae), a globally important pest of pome fruits and walnuts. The gene structure of the C. pomonella Masc ortholog, CpMasc, is similar to B. mori Masc. However, unlike B. mori, we identified 14 splice variants of CpMasc in the available transcriptomes. Subsequent screening for sex specificity and genetic variation using publicly available data and RT-PCR revealed three male-specific splice variants. Then qPCR analysis of these variants revealed sex-biased expression showing a peak only in early male embryos. Knockdown of CpMasc by RNAi during early embryogenesis resulted in a shift from male-to female-specific splicing of the C. pomonella doublesex (Cpdsx) gene, its downstream effector, in ZZ embryos, leading to a strongly female-biased sex ratio. These data clearly demonstrate that CpMasc functions as a masculinizing gene in the sex-determining cascade of C. pomonella. Our study also showed that CpMasc transcripts are provided maternally, as they were detected in unfertilized eggs after oviposition and in mature eggs dissected from virgin females. This finding is unique, as maternal provision of mRNA has rarely been studied in Lepidoptera.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10603 - Genetics and heredity (medical genetics to be 3)

Project

<a href="/en/project/GA20-13784S" target="_blank" >GA20-13784S: Chromosomal and molecular mechanisms of sex determination in Lepidoptera</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Insect Biochemistry and Molecular Biology

ISSN

0965-1748

e-ISSN

1879-0240

Volume of the periodical

160

Issue of the periodical within the volume

SEP 01

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

103991

UT code for WoS article

001053153200001

EID of the result in the Scopus database

2-s2.0-85166643232