Development of recombinant West Nile virus expressing mCherry reporter protein

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F23%3A00574469" target="_blank" >RIV/60077344:_____/23:00574469 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/23:00131190 RIV/00027162:_____/23:N0000085

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0166093423000691?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0166093423000691?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jviromet.2023.114744" target="_blank" >10.1016/j.jviromet.2023.114744</a>

Result language

angličtina

Original language name

Development of recombinant West Nile virus expressing mCherry reporter protein

Original language description

West Nile virus (WNV) is transmitted to humans and animals by a mosquito and enters the central nervous system, leading to lethal encephalitis. Reporter viruses expressing fluorescent proteins enable detection of infected cells in vitro and in vivo, facilitating evaluation of the dynamics of viral infection, and the development of diagnostic or therapeutic methods. In this study, we developed a method for production of a recombinant replication-competent WNV expressing mCherry fluorescent protein. The expression of mCherry was observed in viral antigen-positive cells in vitro and in vivo, but the growth of the reporter WNV was reduced as compared to the parental WNV. The expression of mCherry was stable during 5 passages in reporter WNV-infected culture cells. Neurological symptoms were observed in mice inoculated intracranially with the reporter WNV. The reporter WNV expressing mCherry will facilitate research into WNV replication in mouse brains.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Virological Methods

ISSN

0166-0934

e-ISSN

1879-0984

Volume of the periodical

317

Issue of the periodical within the volume

APR

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

5

Pages from-to

114744

UT code for WoS article

001008384800001

EID of the result in the Scopus database

2-s2.0-85154069223