Two novel <i>Bartonella</i> (sub)species isolated from edible dormice (<i>Glis glis</i>): hints of cultivation stress-induced genomic changes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F23%3A00580236" target="_blank" >RIV/60077344:_____/23:00580236 - isvavai.cz</a>
Result on the web
<a href="https://www.frontiersin.org/articles/10.3389/fmicb.2023.1289671/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fmicb.2023.1289671/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fmicb.2023.1289671" target="_blank" >10.3389/fmicb.2023.1289671</a>
Alternative languages
Result language
angličtina
Original language name
Two novel <i>Bartonella</i> (sub)species isolated from edible dormice (<i>Glis glis</i>): hints of cultivation stress-induced genomic changes
Original language description
Bartonelloses are neglected emerging infectious diseases caused by facultatively intracellular bacteria transmitted between vertebrate hosts by various arthropod vectors. The highest diversity of Bartonella species has been identified in rodents. Within this study we focused on the edible dormouse (Glis glis), a rodent with unique life-history traits that often enters households and whose possible role in the epidemiology of Bartonella infections had been previously unknown. We identified and cultivated two distinct Bartonella sub(species) significantly diverging from previously described species, which were characterized using growth characteristics, biochemical tests, and various molecular techniques including also proteomics. Two novel (sub)species were described: Bartonella grahamii subsp. shimonis subsp. nov. and Bartonella gliris sp. nov. We sequenced two individual strains per each described (sub)species. During exploratory genomic analyses comparing two genotypes ultimately belonging to the same species, both factually and most importantly even spatiotemporally, we noticed unexpectedly significant structural variation between them. We found that most of the detected structural variants could be explained either by prophage excision or integration. Based on a detailed study of one such event, we argue that prophage deletion represents the most probable explanation of the observed phenomena. Moreover, in one strain of Bartonella grahamii subsp. shimonis subsp. nov. we identified a deletion related to Bartonella Adhesin A, a major pathogenicity factor that modulates bacteria-host interactions. Altogether, our results suggest that even a limited number of passages induced sufficient selective pressure to promote significant changes at the level of the genome.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Microbiology
ISSN
1664-302X
e-ISSN
1664-302X
Volume of the periodical
14
Issue of the periodical within the volume
NOV
Country of publishing house
CH - SWITZERLAND
Number of pages
13
Pages from-to
1289671
UT code for WoS article
001110460400001
EID of the result in the Scopus database
2-s2.0-85177996747