Two novel Bartonella (sub)species isolated from edible dormice (Glis glis): hints of cultivation stress-induced genomic changes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3A_____%2F23%3AN0000008" target="_blank" >RIV/60162694:_____/23:N0000008 - isvavai.cz</a>

Alternative codes found

RIV/61383082:_____/23:00001288 RIV/60460709:41210/23:96595 RIV/00216224:14310/23:00133010 RIV/62690094:18470/23:50020820 and 3 more

Result on the web

<a href="https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1289671/full" target="_blank" >https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1289671/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fmicb.2023.1289671" target="_blank" >10.3389/fmicb.2023.1289671</a>

Result language

angličtina

Original language name

Two novel Bartonella (sub)species isolated from edible dormice (Glis glis): hints of cultivation stress-induced genomic changes

Original language description

Bartonelloses are neglected emerging infectious diseases caused by facultatively intracellular bacteria transmitted between vertebrate hosts by various arthropod vectors. The highest diversity of Bartonella species has been identified in rodents. Within this study we focused on the edible dormouse (Glis glis), a rodent with unique life-history traits that often enters households and whose possible role in the epidemiology of Bartonella infections had been previously unknown. We identified and cultivated two distinct Bartonella sub(species) significantly diverging from previously described species, which were characterized using growth characteristics, biochemical tests, and various molecular techniques including also proteomics. Two novel (sub)species were described: Bartonella grahamii subsp. shimonis subsp. nov. and Bartonella gliris sp. nov. We sequenced two individual strains per each described (sub)species. During exploratory genomic analyses comparing two genotypes ultimately belonging to the same species, both factually and most importantly even spatiotemporally, we noticed unexpectedly significant structural variation between them. We found that most of the detected structural variants could be explained either by prophage excision or integration. Based on a detailed study of one such event, we argue that prophage deletion represents the most probable explanation of the observed phenomena. Moreover, in one strain of Bartonella grahamii subsp. shimonis subsp. nov. we identified a deletion related to Bartonella Adhesin A, a major pathogenicity factor that modulates bacteria-host interactions. Altogether, our results suggest that even a limited number of passages induced sufficient selective pressure to promote significant changes at the level of the genome.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/VJ01010107" target="_blank" >VJ01010107: Extending and characterizing the collection of high-risk and risk biological causative agents of dangerous infectious diseases and implementing new procedures for working with them</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Microbiology

ISSN

1664-302X

e-ISSN

1664-302X

Volume of the periodical

14

Issue of the periodical within the volume

15.11.2023 (1289671)

Country of publishing house

CH - SWITZERLAND

Number of pages

13

Pages from-to

nestrankovano

UT code for WoS article

001110460400001

EID of the result in the Scopus database

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