A thylakoid biogenesis BtpA protein is required for the initial step of tetrapyrrole biosynthesis in cyanobacteria
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00598342" target="_blank" >RIV/60077344:_____/24:00598342 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/24:00598265 RIV/60076658:12310/24:43908293
Result on the web
<a href="https://nph.onlinelibrary.wiley.com/doi/epdf/10.1111/nph.19397" target="_blank" >https://nph.onlinelibrary.wiley.com/doi/epdf/10.1111/nph.19397</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/nph.19397" target="_blank" >10.1111/nph.19397</a>
Alternative languages
Result language
angličtina
Original language name
A thylakoid biogenesis BtpA protein is required for the initial step of tetrapyrrole biosynthesis in cyanobacteria
Original language description
Biogenesis of the photosynthetic apparatus requires complicated molecular machinery, individual components of which are either poorly characterized or unknown. The BtpA protein has been described as a factor required for the stability of photosystem I (PSI) in cyanobacteria, however, how the BtpA stabilized PSI remains unexplained.To clarify the role of BtpA, we constructed and characterized the btpA-null mutant (Delta btpA) in the cyanobacterium Synechocystis sp. PCC 6803. The mutant contained only c. 1% of chlorophyll and nearly no thylakoid membranes. However, this strain, growing only in the presence of glucose, was genetically unstable and readily generated suppressor mutations that restore the photoautotrophy.Two suppressor mutations were mapped into the hemA gene encoding glutamyl-tRNA reductase (GluTR) the first enzyme of tetrapyrrole biosynthesis. Indeed, the GluTR was not detectable in the Delta btpA mutant and the suppressor mutations restored biosynthesis of tetrapyrroles and photoautotrophy by increased GluTR expression or by improved GluTR stability/processivity. We further demonstrated that GluTR associates with a large BtpA oligomer and that BtpA is required for the stability of GluTR.Our results show that the BtpA protein is involved in the biogenesis of photosystems at the level of regulation of tetrapyrrole biosynthesis.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10605 - Developmental biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
New Phytologist
ISSN
0028-646X
e-ISSN
1469-8137
Volume of the periodical
241
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
1236-1249
UT code for WoS article
001107509000001
EID of the result in the Scopus database
2-s2.0-85177479911