Exploring the transcriptomic profile of human monkeypox virus via CAGE and native RNA sequencing approaches
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00598826" target="_blank" >RIV/60077344:_____/24:00598826 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/24:00138727 RIV/00027162:_____/24:N0000101
Result on the web
<a href="https://doi.org/10.1128/msphere.00356-24" target="_blank" >https://doi.org/10.1128/msphere.00356-24</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1128/msphere.00356-24" target="_blank" >10.1128/msphere.00356-24</a>
Alternative languages
Result language
angličtina
Original language name
Exploring the transcriptomic profile of human monkeypox virus via CAGE and native RNA sequencing approaches
Original language description
In this study, we employed short- and long-read sequencing technologies to delineate the transcriptional architecture of the human monkeypox virus and to identify key regulatory elements that govern its gene expression. Specifically, we conducted a transcriptomic analysis to annotate the transcription start sites (TSSs) and transcription end sites (TESs) of the virus by utilizing Cap Analysis of gene expression sequencing on the Illumina platform and direct RNA sequencing on the Oxford Nanopore technology device. Our investigations uncovered significant complexity in the use of alternative TSSs and TESs in viral genes. In this research, we also detected the promoter elements and poly(A) signals associated with the viral genes. Additionally, we identified novel genes in both the left and right variable regions of the viral genome.IMPORTANCEGenerally, gaining insight into how the transcription of a virus is regulated offers insights into the key mechanisms that control its life cycle. The recent outbreak of the human monkeypox virus has underscored the necessity of understanding the basic biology of its causative agent. Our results are pivotal for constructing a comprehensive transcriptomic atlas of the human monkeypox virus, providing valuable resources for future studies.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
mSphere
ISSN
2379-5042
e-ISSN
2379-5042
Volume of the periodical
9
Issue of the periodical within the volume
9
Country of publishing house
US - UNITED STATES
Number of pages
22
Pages from-to
e0035624
UT code for WoS article
001299806300004
EID of the result in the Scopus database
2-s2.0-85205083549