V9 Hypervariable Region Metabarcoding Primers for Euglenozoa and Metamonada
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00599967" target="_blank" >RIV/60077344:_____/24:00599967 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/24:43908777 RIV/00216208:11310/24:10487026
Result on the web
<a href="https://doi.org/10.1002/edn3.70022" target="_blank" >https://doi.org/10.1002/edn3.70022</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/edn3.70022" target="_blank" >10.1002/edn3.70022</a>
Alternative languages
Result language
angličtina
Original language name
V9 Hypervariable Region Metabarcoding Primers for Euglenozoa and Metamonada
Original language description
Short amplicon sequencing is a commonly used method to study the diversity of organisms in various habitats. The hypervariable regions of the small subunit rRNA gene (18S rDNA) are the most general barcodes for eukaryotes, which can provide detailed taxonomic information across a wide range of eukaryotic diversity. However, some organisms are often missed by universal primers, which have difficulty amplifying their barcodes. In this study, specific primers were designed for the amplification of the highly diverse 18S-V9 region of the Euglenozoa and Metamonada groups. The performance of the newly designed primers-V9Eug and V9Meta-was compared with the universal V9 primer on cultured communities derived from a range of freshwater environments of the Soos Natural Reserve and the Slavkov Forest in the Czech Republic. The V9Eug primer was more specific with Euglenozoa representing 91.8% of reads and 57.0% of OTUs, while the V9Meta primer showed lower specificity with only 48.4% of reads and 19.7% of OTUs assigned to Metamonada. Both the Euglenozoa and Metamonada primer pairs significantly improved recovery of their target groups compared to the universal V9 primer pair, detecting 2.7 and 1.8 times more OTUs, respectively. These results provide a more sensitive protocol for studying the diversity of these eukaryotic taxa.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Environmental DNA
ISSN
2637-4943
e-ISSN
2637-4943
Volume of the periodical
6
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
e70022
UT code for WoS article
001335902500001
EID of the result in the Scopus database
2-s2.0-85206369137