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Archaeal community dynamics in biogas fermentation at various temperatures assessed by mcrA amplicon sequencing using different primer pairs

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F21%3A43920352" target="_blank" >RIV/62156489:43210/21:43920352 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216224:14310/21:00122528

  • Result on the web

    <a href="https://doi.org/10.1007/s11274-021-03152-w" target="_blank" >https://doi.org/10.1007/s11274-021-03152-w</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s11274-021-03152-w" target="_blank" >10.1007/s11274-021-03152-w</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Archaeal community dynamics in biogas fermentation at various temperatures assessed by mcrA amplicon sequencing using different primer pairs

  • Original language description

    In this study, the taxonomic and functional diversity of methanogenic archaea in two parallel 120 l fermenters operated at different temperatures and fed with maize silage was estimated by mcrA metabarcoding analysis using two typical primer pairs (ML and MLA) amplifying part of the functional methyl coenzyme M reductase (mcrA) gene. The alpha diversity indices showed that the ML primer pair detected a higher Operational Taxonomic Unit (OTU) abundance compared to the MLA primer pair and methanogen diversity was significantly lower in the 60 oC fermenters. The beta diversity analysis showed the methanogenic community clustered together at 50 oC and 40o and was statistically different from the 60 oC community. Similar, to alpha diversity, beta diversity was also significantly different between primer pairs. At all temperatures analysed, the primer pairs showed a different abundance of the different methanogenic OTUs, e.g. more OTUs relative to Methanoculleus sp. with the ML primer pair, and more OTUs corresponding to Methanobacterium sp. with the MLA primer pair. Moreover, OTUs corresponding to Methanosphaera sp. and Methanobrevibacter sp. were found only by using ML primer pair, while the MLA primer pair detected sequences corresponding to Methanothrix sp.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10606 - Microbiology

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    World Journal of Microbiology and Biotechnology

  • ISSN

    0959-3993

  • e-ISSN

  • Volume of the periodical

    37

  • Issue of the periodical within the volume

    11

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    11

  • Pages from-to

    188

  • UT code for WoS article

    000703872700001

  • EID of the result in the Scopus database

    2-s2.0-85116368863