Identification of 30 transition fibre proteins in Trypanosoma brucei reveals a complex and dynamic structure

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00603911" target="_blank" >RIV/60077344:_____/24:00603911 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1242/jcs.261692" target="_blank" >https://doi.org/10.1242/jcs.261692</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1242/jcs.261692" target="_blank" >10.1242/jcs.261692</a>

Result language

angličtina

Original language name

Identification of 30 transition fibre proteins in Trypanosoma brucei reveals a complex and dynamic structure

Original language description

Transition fibres and distal appendages surround the distal end of mature basal bodies and are essential for ciliogenesis, but only a few of the proteins involved have been identified and functionally characterised. Here, through genome-wide analysis, we have identified 30 transition fibre proteins (TFPs) and mapped their arrangement in the flagellated eukaryote Trypanosoma brucei. We discovered that TFPs are recruited to the mature basal body before and after basal body duplication, with differential expression of five TFPs observed at the assembling new flagellum compared to the existing fixed-length old flagellum. RNAi-mediated depletion of 17 TFPs revealed six TFPs that are necessary for ciliogenesis and a further three TFPs that are necessary for normal flagellum length. We identified nine TFPs that had a detectable orthologue in at least one basal body-forming eukaryotic organism outside of the kinetoplastid parasites. Our work has tripled the number of known transition fibre components, demonstrating that transition fibres are complex and dynamic in their composition throughout the cell cycle, which relates to their essential roles in ciliogenesis and flagellum length regulation.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10601 - Cell biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Cell Science

ISSN

0021-9533

e-ISSN

1477-9137

Volume of the periodical

137

Issue of the periodical within the volume

10

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

jcs261692

UT code for WoS article

001266940200004

EID of the result in the Scopus database

2-s2.0-85195227059