Intricate balance of dually-localized catalase in /Leptomonas seymouri/ (Kinetoplastea: Trypanosomatidae) modulates infectivity

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00604262" target="_blank" >RIV/60077344:_____/24:00604262 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1016/j.ijpara.2024.04.007" target="_blank" >https://doi.org/10.1016/j.ijpara.2024.04.007</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijpara.2024.04.007" target="_blank" >10.1016/j.ijpara.2024.04.007</a>

Result language

angličtina

Original language name

Intricate balance of dually-localized catalase in /Leptomonas seymouri/ (Kinetoplastea: Trypanosomatidae) modulates infectivity

Original language description

Nearly all aerobic organisms are equipped with catalases, powerful enzymes scavenging hydrogen peroxide and facilitating defense against harmful reactive oxygen species. In trypanosomatids, this enzyme was not present in the common ancestor, yet it had been independently acquired by different lineages of monoxenous trypanosomatids from different bacteria at least three times. This observation posited an obvious question: why was catalase so sought after if many trypanosomatid groups do just fine without it? In this work, we analyzed subcellular localization and function of catalase in Leptomonas seymouri. We demonstrated that this enzyme is present in the cytoplasm and a subset of glycosomes, and that its cytoplasmic retention is H2O2-dependent. The ablation of catalase in this parasite is not detrimental in vivo, while its overexpression resulted in a substantially higher parasite load in the experimental infection of Dysdercus peruvianus. We propose that the capacity of studied flagellates to modulate the catalase activity in the midgut of its insect host facilitates their development and protects them from oxidative damage at elevated temperatures. CO 2024 The Author(s). Published by Elsevier Ltd on behalf of Australian Society for Parasitology. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/GA21-09283S" target="_blank" >GA21-09283S: Catalase: to be or not to be in two hosts</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal for Parasitology

ISSN

0020-7519

e-ISSN

1879-0135

Volume of the periodical

54

Issue of the periodical within the volume

8-9

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

10

Pages from-to

391-400

UT code for WoS article

001255604200001

EID of the result in the Scopus database

2-s2.0-85191821063