A Protocol for GC-MS Profiling of Chiral Secondary Amino Acids

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00604733" target="_blank" >RIV/60077344:_____/25:00604733 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/protocol/10.1007/978-1-0716-4334-1_11" target="_blank" >https://link.springer.com/protocol/10.1007/978-1-0716-4334-1_11</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-0716-4334-1_11" target="_blank" >10.1007/978-1-0716-4334-1_11</a>

Result language
angličtina
Original language name
A Protocol for GC-MS Profiling of Chiral Secondary Amino Acids
Original language description
A simple analytical workflow is described for gas chromatographic-mass spectrometric (GC-MS)-based chiral profiling of secondary amino acids (AAs) in biological matrices. The sample preparation is carried out directly in aqueous biological sample extracts and involves in situ heptafluorobutyl chloroformate (HFBCF) derivatization-liquid-liquid microextraction of nonpolar products into hexane phase followed by subsequent formation of the corresponding methylamides from the HFB esters by direct treatment with methylamine reagent solution. The (O, N) HFB-butoxycarbonyl-methylamide AA products (HFBOC-MA) are separated on a Chirasil-L-Val capillary column and quantitatively measured by GC-MS operated in selected ion monitoring (SIM) mode. The protocol includes 12 simple pipetting steps and covers the quantitative analysis of 8L, D pairs of secondary amino acids, including proline, isomeric 3-, 4-hydroxyprolines, pipecolic acid, nipecotic acid, azetidine-2-carboxylic acid, and cis- and trans-5-hydroxy-L-pipecolic acid using 13C5L-proline as an internal standard. The individual analytical steps are commented on and explained, with emphasis on the chiral GC-MS analysis of secondary amino acids in human urine, serum, and peptide hydrolysate samples.
Czech name
—
Czech description
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Project
<a href="/en/project/GA23-06600S" target="_blank" >GA23-06600S: New approaches in targeted metabolomic analysis of unstable metabolites with oxo group: labeling with quaternary ammonium and stable isotopes</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Book/collection name
Metabolomic Profiling: Methods and Protocols, Methods in Molecular Biology
ISBN
978-1-0716-4333-4
Number of pages of the result
15
Pages from-to
205-219
Number of pages of the book
286
Publisher name
Humana Press
Place of publication
New York, NY
UT code for WoS chapter
001555691800012

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