Effect of selected Indonesian plants on Giardia intestinalis in an experimental in vitro model

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00616973" target="_blank" >RIV/60077344:_____/25:00616973 - isvavai.cz</a>

Alternative codes found

RIV/61389030:_____/25:00616973 RIV/00216224:14310/25:00144259 RIV/61989592:15310/25:73634833

Result on the web

<a href="https://doi.org/10.1186/s12906-025-04755-8" target="_blank" >https://doi.org/10.1186/s12906-025-04755-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12906-025-04755-8" target="_blank" >10.1186/s12906-025-04755-8</a>

Result language

angličtina

Original language name

Effect of selected Indonesian plants on Giardia intestinalis in an experimental in vitro model

Original language description

Background nThis study evaluated in vitro antigiardial activity in four Indonesian plants (Archidendron fagifolium, Diospyros sumatrana, Piper betle and Shorea sumatrana) extracted in methanol, methanol-tetrahydrofuran, and water. These plants exhibiting promising anti-parasitic activity were selected on the basis of collected behavioral data and their ability to decrease parasite load in Sumatran orangutans. Extracts of Arabidopsis thaliana, a plant routinely used as a laboratory model in research, were used as a negative control.nMethodsnPlant extracts of different concentrations (400, 100, 25 and 6.25 mu g/ml) and metronidazole (100 mu g/ml), a standard giardicidal drug, were incubated with 25,000 trophozoites per milliliter of growth medium in 12-well tissue culture plates under anaerobic conditions for 72 h. Cultures were counted in a hemocytometer using a light microscope and then statistically evaluated. The cytotoxicity of the extracts was determined by the MTT Cell Viability Assay.nResultsnWe recorded a statistically-significant decrease in giardia trophozoites in two extracts: the methanolic extract of A. fagifolium (Kruskal-Wallis: p = 0.013) and the aqueous extract of P. betle (Kruskal-Wallis: p = 0.002). Effective concentrations of 400 mu g/ml and 100 mu g/ml, respectively, were revealed. Cytotoxicity was not demonstrated.nConclusionsnThe results of our study show that extracts of A. fagifolium and P. betle have potential as an alternative treatment of G. intestinalis infection. This is the first scientific proof of the biological activity of A. fagifolium, confirming the assumption that orangutans use this plant (as well as P. betle) for self-medication.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30107 - Medicinal chemistry

Project

<a href="/en/project/GA23-06571S" target="_blank" >GA23-06571S: What orang-utans can teach us: plants used for self-medication as a potential source of bioactive substances with amoebicidal and antigiardial effects</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BMC Complementary Medicine and Therapies

ISSN

2662-7671

e-ISSN

2662-7671

Volume of the periodical

25

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

22

UT code for WoS article

001406050800001

EID of the result in the Scopus database

2-s2.0-85216718146