Immunostimulant effect of heat-inactivated Mycobacterium bovis in mice challenged with vector-borne pathogens

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00619123" target="_blank" >RIV/60077344:_____/25:00619123 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1016/j.vaccine.2025.127076" target="_blank" >https://doi.org/10.1016/j.vaccine.2025.127076</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.vaccine.2025.127076" target="_blank" >10.1016/j.vaccine.2025.127076</a>

Result language

angličtina

Original language name

Immunostimulant effect of heat-inactivated Mycobacterium bovis in mice challenged with vector-borne pathogens

Original language description

Trained immunity is defined as an enhanced state of the innate system which leads to an improved immune response against related or non-related pathogens. Bacillus Calmette-Gue<acute accent>rin (BCG) vaccine, a live attenuated Mycobacterium bovis strain, is currently one of the main inductors of trained immunity. The objective of the present study was to evaluate the protective effects of heat-inactivated M. bovis (HIMB) against Plasmodium berghei and Borrelia burgdorferi and characterize the immunological mechanisms involved. BALB/c and C3H/HeN mice were randomly assigned in similar number to either immunized group receiving two oral doses of HIMB with a 4-week interval, or control group treated with PBS. All the BALB/c mice were intraperitoneally infected with P. berghei while the C3H/HeN mice were subcutaneously infected with B. burgdorferi. Pathogen burden was significantly reduced in both immunized groups when compared to controls. The number of macrophages significantly decreased in the liver or in the spleen of the mice that had been immunized prior to the challenge with P. berghei or B. burgdorferi, respectively. Furthermore, the immunized groups showed an apparent upregulation of IFN-gamma, TNF-alpha and IL-1 alpha in the liver (P. berghei challenge) or a significant increase in IL-1 alpha producing cells in the spleen (B. burgdorferi challenge). Our findings suggest that oral immunization with heat-inactivated mycobacteria limits pathogen burden through stimulation of the innate immune response in two vector-borne diseases in mice.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30102 - Immunology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Vaccine

ISSN

0264-410X

e-ISSN

1873-2518

Volume of the periodical

53

Issue of the periodical within the volume

APR

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

127076

UT code for WoS article

001464783600001

EID of the result in the Scopus database

2-s2.0-105001700844