Enhanced RNAi does not provide efficient innate antiviral immunity in mice
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00642229" target="_blank" >RIV/60077344:_____/25:00642229 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/25:00642229 RIV/60076658:12310/25:43909734 RIV/00216224:14310/25:00140568 RIV/00027162:_____/25:N0000012
Result on the web
<a href="https://academic.oup.com/nar/article/53/1/gkae1288/7945392" target="_blank" >https://academic.oup.com/nar/article/53/1/gkae1288/7945392</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/nar/gkae1288" target="_blank" >10.1093/nar/gkae1288</a>
Alternative languages
Result language
angličtina
Original language name
Enhanced RNAi does not provide efficient innate antiviral immunity in mice
Original language description
In RNA interference (RNAi), long double-stranded RNA is cleaved by the Dicer endonuclease into small interfering RNAs (siRNAs), which guide degradation of complementary RNAs. While RNAi mediates antiviral innate immunity in plants and many invertebrates, vertebrates have adopted a sequence-independent response and their Dicer produces siRNAs inefficiently because it is adapted to process small hairpin microRNA precursors in the gene-regulating microRNA pathway. Mammalian endogenous RNAi is thus a rudimentary pathway of unclear significance. To investigate its antiviral potential, we modified the mouse Dicer locus to express a truncated variant (Dicer ΔHEL1 ) known to stimulate RNAi and we analyzed how Dicer ΔHEL1/wt mice respond to four RNA viruses: coxsackievirus B3 and encephalomyocarditis virus from Picornaviridae, tick-borne encephalitis virus from Flaviviridae, and lymphocytic choriomeningitis virus (LCMV) from Arenaviridae. Increased Dicer activity in Dicer ΔHEL1/wt mice did not elicit any antiviral effect, supporting an insignificant antiviral function of endogenous mammalian RNAi in vivo. However, we also observed that sufficiently high expression of DicerΔHEL1 suppressed LCMV in embryonic stem cells and in a transgenic mouse model. Altogether, mice with increased Dicer activity offer a new benchmark for identifying and studying viruses susceptible to mammalian RNAi in vivo.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10607 - Virology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nucleic Acids Research
ISSN
0305-1048
e-ISSN
1362-4962
Volume of the periodical
53
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
18
Pages from-to
gkae1288
UT code for WoS article
001391535800001
EID of the result in the Scopus database
2-s2.0-85214572319