Splice site diversity and abundance of noncanonical introns in diplonemids (Diplonemea, Euglenozoa)

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00644363" target="_blank" >RIV/60077344:_____/25:00644363 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/25:00644363 RIV/61988987:17310/25:A2603CWF RIV/60076658:12310/25:43910289
Result on the web
<a href="https://rnajournal.cshlp.org/content/31/12/1826.full" target="_blank" >https://rnajournal.cshlp.org/content/31/12/1826.full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1261/rna.080641.125" target="_blank" >10.1261/rna.080641.125</a>

Result language
angličtina
Original language name
Splice site diversity and abundance of noncanonical introns in diplonemids (Diplonemea, Euglenozoa)
Original language description
Noncoding introns are a unifying feature of protein-coding genes in virtually all extant eukaryotes, with most lineages following the canonical intron structure. However, euglenozoans, unicellular flagellates that include free-living euglenids, human pathogenic kinetoplastids, and highly diverse and abundant marine diplonemids, are a notable exception. Euglenozoan genomes range from extremely intron-poor kinetoplastids to euglenid genomes containing both canonical and noncanonical introns. Here, we present a comprehensive analysis of splice sites and spliceosomal components in six species of understudied diplonemids. All diplonemids examined contain a nearly complete set of spliceosomal snRNP components, indicating the presence of a functional U2-type spliceosome. However, the majority of introns in the hemistasiid diplonemids Artemidia motanka and Namystynia karyoxenos are noncanonical and lack conserved GT-AG terminal dinucleotides typical for U2-type introns. These noncanonical introns are capable of extensive base-pairing, which brings intron ends into close proximity. Thus, while the splicing apparatus is conserved in diplonemids, the splice sites are highly variable among individual species.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30310 - Parasitology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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