Cultivation, genomics, and giant viruses of a ubiquitous and heterotrophic freshwater cryptomonad

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00647074" target="_blank" >RIV/60077344:_____/25:00647074 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/25:43910593

Result on the web

<a href="https://doi.org/10.1093/ismejo/wraf271" target="_blank" >https://doi.org/10.1093/ismejo/wraf271</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/ismejo/wraf271" target="_blank" >10.1093/ismejo/wraf271</a>

Result language

angličtina

Original language name

Cultivation, genomics, and giant viruses of a ubiquitous and heterotrophic freshwater cryptomonad

Original language description

Heterotrophic nanoflagellates are the chief agents of bacterivory in the aquatic microbial loop but remain underrepresented in culture collections and in genomic databases. We isolated and characterized a representative of the previously uncultured freshwater Cryptomonad Group 1 (CRY1a) lineage using a genome-streamlined, ultra-small and abundant microbe Planktophila versatilis as a prey and Catalyzed Reporter Deposition-Fluorescence in situ Hybridization (CARD-FISH) probe-based screening. This isolate, Tyrannomonas regina, is one of the most dominant ubiquitous heterotrophic cryptomonads in freshwaters. It is a small heterotrophic nanoflagellate (ca. 3-5 mu m) and has the smallest genome of any cryptomonad sequenced thus far. The compact genome (ca. 69 Mb) revealed no traces of a photosynthetic lifestyle, consistent with its phylogenomic placement as a sister clade to cryptophytes that are characterized by the acquisition of a red-algal symbiont. Moreover, in comparison to its photosynthetic counterparts, its genome presents substantially lower repeat content and endogenous viral elements. Genomes of two giant viruses, Tyrannovirus reginensis GV1 and GV2, were also recovered from the same culture and represent a viral genus that has been described so far solely by metagenome-recovered genomes. Collectively, these findings provide insights into genomic ancestry and evolution, widespread ecological impact, and interactions of an elusive protist lineage and illustrate the advantages of culture-centric approaches towards unfolding complex tapestries of life in the microbial world.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10617 - Marine biology, freshwater biology, limnology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

The ISME Journal

ISSN

1751-7362

e-ISSN

1751-7370

Volume of the periodical

19

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

wraf271

UT code for WoS article

001649991100001

EID of the result in the Scopus database

2-s2.0-105026364181