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Limitations in reliability of fire blight (Erwinia amylovora) diagnostic methods

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077352%3A_____%2F05%3A00027719" target="_blank" >RIV/60077352:_____/05:00027719 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Limitations in reliability of fire blight (Erwinia amylovora) diagnostic methods

  • Original language description

    A total of 20 Erwinia amylovora-like (Ea-like) isolates originating from symptomatic samples of fire blight host plant were analysed. 14of them rected negatively in immunochemical tests. Six-like isolates reactted positively with a polyclonal antibody PTA-ELISA (Loewe,Co., Germany) and five of them gave positive reaction indirect IF test (Adgen Co., Scotland). In hypersensitivity tets in tabacco, all Ea-like isolates were negative.

  • Czech name

    Stanovení spolehlivosti diagnostických metod pro detekci spály růžovitých rostlin (Erwinia amylovora)

  • Czech description

    Bylo analyzováno celkem 20 domnělých kmenů Erwinia amylovora. 14 z nich reagovalo negativně v imunochemických testech. 6 domnělých Ea kmenů reagovalo pozitivně v PT-ELISA a 5 z nich vykazovalo pozitivní reakce v nepřímém IF testu. Po optimalizaci PCR v něm všechny domnělé Ea kmeny reagovaly negativně.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2005

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Phytopathologia Polonica

  • ISSN

    1230-0462

  • e-ISSN

  • Volume of the periodical

    35

  • Issue of the periodical within the volume

    -

  • Country of publishing house

    PL - POLAND

  • Number of pages

    4

  • Pages from-to

    69-72

  • UT code for WoS article

  • EID of the result in the Scopus database