Amplified Ribosomal DNA Restriction Analysis: experimental approach and procedures.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077379%3A_____%2F02%3A61033052" target="_blank" >RIV/60077379:_____/02:61033052 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Amplified Ribosomal DNA Restriction Analysis: experimental approach and procedures.
Original language description
We adapted the amplified ribosomal DNA restriction analysis (ARDRA) to examine the bacterial communities associated with the soil dwelling larvae of Penthetria holosericea (Diptera) and with vermicompost and earthworm (Eisenia andrei) gut content. Following DNA isolation and PCR amplification of the 16S rDNA, three restriction enzymes were used to generate ARDRA patterns. Resulting fragments were separated in a 1,5% agarose gel. When the universal eubacterial primers 63f and 1387r were used, the ARDRA patterns proved to be complex and difficult to resolve, although some minor differences were noted. The use of taxon specific PCR primers resulted in less complicated patterns and more differences in the band composition. The method is fast, cheap and useful in the characterization of bacterial communities.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
EH - Ecology - communities
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2002
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Život v pode. 3. medzinárodný seminár, sborník příspěvků na CD.
ISBN
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ISSN
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e-ISSN
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Number of pages
3
Pages from-to
5-7
Publisher name
Československá spoločnosť mikrobiologická
Place of publication
Bratislava
Event location
Bratislava [SK]
Event date
Jan 29, 2002
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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