Simple and rapid procedure for at the determination of individual free fatty acids in serum.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077395%3A_____%2F02%3A60023024" target="_blank" >RIV/60077395:_____/02:60023024 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Simple and rapid procedure for at the determination of individual free fatty acids in serum.

Original language description

A microscale procedure for a rapid extraction, re-extraction and methylation of serum or plasma free fatty acids (FFAs) is described. There is no necessity to evaporate extracting solvent or heat the reaction medium to prepare corresponding methyl estersfor gas chromatographic analysis. The approach comprises three consecutive steps, carried out in two reaction vials, during which the neutral lipids are removed and the free fatty acids re-extracted and subsequently methylated. Methylation succeds uponusing methyl chloroformate in combination with a non-smelling organic base within seconds. The method proved to be highly reproducible, with intraassay variation coefficients being less than 5 % for the major serum fatty acids.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2002

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytica Chimica Acta

ISSN

0003-2670

e-ISSN

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Volume of the periodical

465

Issue of the periodical within the volume

N/A

Country of publishing house

US - UNITED STATES

Number of pages

7

Pages from-to

433-439

UT code for WoS article

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EID of the result in the Scopus database

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