Production of Polyclonal Antibodies to the Recombinant Potato virus M (PVM) Non-structural Triple Gene Block Protein 1 and Coat Protein

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60109807%3A_____%2F12%3A%230000202" target="_blank" >RIV/60109807:_____/12:#0000202 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Production of Polyclonal Antibodies to the Recombinant Potato virus M (PVM) Non-structural Triple Gene Block Protein 1 and Coat Protein

Original language description

The genes encoding the coat protein (CP) and triple gene block protein 1 (TGBp1) of Potato virus M (PVM) were cloned into expression vector pET- 45b(+) (N-terminal 6xHis tag) and expressed in E. coli Rosetta gami-2(DE3). The purified recombinant antigenswere used for raising polyclonal antibodies. The antibodies against recombinant CP were successfully used in Western blot analysis, plate-trapped ELISA and DAS-ELISA as a coating for PVM detection in infected potato leaf samples. The antibodies againstrecombinant non-structural protein detected the TGBp1 only in Western blot analysis. This is the first report of the production of polyclonal antibodies against recombinant coat protein and TGBp1 of PVM and their use for detecting the virus.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/1M06030" target="_blank" >1M06030: Functional genomics and proteomics for crop improvement</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

J Phytopathol

ISSN

1439-0434

e-ISSN

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Volume of the periodical

160

Issue of the periodical within the volume

2012.01886

Country of publishing house

DE - GERMANY

Number of pages

3

Pages from-to

130-132

UT code for WoS article

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EID of the result in the Scopus database

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