Polyclonal Antibodies to a Recombinant Coat Protein of Potato Virus A.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F02%3A56023029" target="_blank" >RIV/61389030:_____/02:56023029 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Polyclonal Antibodies to a Recombinant Coat Protein of Potato Virus A.
Original language description
Specific mouse antibodies against a recombinant coat protein (CP) of Potato virus A (PVA) were produced. The PVA CP gene was cloned in an expression vector pMPM4£2. After expression in Escherichia coli the presence of the expressed CP was proved byWestern blot analysis using polyclonal and monoclonal antibodies (MAbs). The expressed CP was purified by centrifugation in CsCl density gradient or on a sucrose cushion. The production of virus-like particles (VLPs) was proved by electron microscopy. The purified CP was used for preparation of a mouse antiserum which had a liter of 1:1024 in ELIS A and reacted specifically in Western blot analysis and indirect plate-trapped antigen ELISA (PTA-ELISA).
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
<a href="/en/project/GA310%2F00%2F0381" target="_blank" >GA310/00/0381: Production of human papillomavirus proteins in transgenic plants: an attempt to develop edible vaccines</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2002
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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