The utilization of molecular markers at selected interspecific hybrids of Solanum species and for the assessment of wild Solanum species subcollection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60109807%3A_____%2F18%3AN0000022" target="_blank" >RIV/60109807:_____/18:N0000022 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The utilization of molecular markers at selected interspecific hybrids of Solanum species and for the assessment of wild Solanum species subcollection

Original language description

The selected wild Solanum species were chosen for crossing by means of traditional breeding methods. These wild species, maintained in genebank of Potato Research Institute in Havlíčkův Brod, are a good source of resistance to various biotic and abiotic factors. Hundreds of hybrids from many combinations, which were arisen by reciprocal crossing procedures (wild species x variety or dihaploid) and parental genotypes were tested by means of PCR methods. We obtained the crossing materials with wild species (S. acaule, S. berthaultii and S. demissum) with potato varieties. Hybridity of progeny after interspecific hybridization was visually checked by morphological traits and RAPD method. The hybridity was found at some hybrids from crossing combinations only. By means of PCR genotypes from seven wild species (S. acaule, S. berthaultii, S. bulbocastanum, S. brevidens, S. demissum, S. pinnatisectum and S. verrucosum) were tested. The plant material was transferred from in vitro conditions to the greenhouse and grown there. The DNA was isolated from these plants by means of GenElute Plant Genomic DNA Miniprep Kit (Sigma). DNA markers were chosen and tested. The selected markers were used for subsequent testing of diploid and tetraploid potato collections. The wild species bearing resistance markers are used for crossing experiments.

Czech name

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Czech description

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Type

J_ost - Miscellaneous article in a specialist periodical

CEP classification

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OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Vědecké práce VÚB Havlíčkův Brod

ISSN

1802-940X

e-ISSN

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Volume of the periodical

24

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

10

Pages from-to

69-78

UT code for WoS article

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EID of the result in the Scopus database

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