The utilization of molecular markers in recent years at selected interspecific hybrids of Solanum species.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60109807%3A_____%2F24%3AN0000007" target="_blank" >RIV/60109807:_____/24:N0000007 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The utilization of molecular markers in recent years at selected interspecific hybrids of Solanum species.

Original language description

The selected wild Solanum species were chosen for crossing by means of traditional breeding methods. These wild species, maintained in genebank of Potato Research Institute in Havlíčkův Brod, are a good source of resistance to various biotic and abiotic factors. Hundreds of hybrids from many combinations, which were arisen by reciprocal crossing procedures (wild species x variety or dihaploid) and parental genotypes were tested by means of PCR methods. We obtained the crossing materials with wild species (S. acaule, S. berthaultii and S. demissum) with potato varieties. Hybridity of progeny after interspecific hybridization was visually checked by morphological traits and RAPD method. The hybridity was found at some hybrids from crossing combinations only. By means of PCR genotypes from seven wild species (S. acaule, S. berthaultii, S. bulbocastanum, S. brevidens, S. demissum, S. pinnatisectum and S. verrucosum) were tested. The plant material was transferred from in vitro conditions to the greenhouse and grown there. The DNA was isolated from these plants by means of GenElute Plant Genomic DNA Miniprep Kit (Sigma). DNA markers were chosen and tested. The selected markers were used for subsequent testing of diploid and tetraploid potato collections. The wild species bearing rresistance genes are used for crossing experiments. The very precise genomic sequence allows for faster and more targeted breeding, as it is easier to find in DNA which crosses with other varieties might be of interest and where the exchange of genetic material between "father" and "mother" should ideally take place. This means that breeders know at an early stage whether a potato has the required properties, such as resistance to specific diseases.

Czech name

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Czech description

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Type

J_ost - Miscellaneous article in a specialist periodical

CEP classification

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OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Vědecké práce VÚB Havlíčkův Brod

ISSN

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e-ISSN

3029-7702

Volume of the periodical

30

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

10

Pages from-to

9-18

UT code for WoS article

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EID of the result in the Scopus database

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