New qPCR-based system for detection of potato viruses PVY and PLRV in aphid vectors.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60109807%3A_____%2F26%3AN0000001" target="_blank" >RIV/60109807:_____/26:N0000001 - isvavai.cz</a>

Result on the web

<a href="https://link.springer.com/article/10.1007/s41348-026-01231-4" target="_blank" >https://link.springer.com/article/10.1007/s41348-026-01231-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s41348-026-01231-4" target="_blank" >10.1007/s41348-026-01231-4</a>

Result language

angličtina

Original language name

New qPCR-based system for detection of potato viruses PVY and PLRV in aphid vectors.

Original language description

The most important potato viruses, PVY and PLRV, are transmitted by vector aphids, particularly Myzus persicae under the climatic conditions of Czechia. The integrated seed-potato protection requires detailed information on virus transmission potential in the potato fields. We developed a new system for simultaneous detection of PVY and PLRV in aphid vectors using two pairs of virus-specific primers targeting conserved genomic regions. The primers function reliably in duplex SYBR Green-based one-step qPCR combined with melting analysis, enabling virus identification by distinct amplicon melting temperatures. The method was validated using diverse virus strains from gene bank collections, artificially infected aphids and aphids collected during field monitoring. The presence of PLRV and PVY was assessed in 446 aphids, including Myzus persicae, Aphis nasturtii and Phorodon humuli, captured using yellow pan traps during 2024. Of these, 42% were PVY-positive, 2.6% PLRV-positive and 1.2% carried mixed infection. The frequency of PVY- and PLRV-positive aphids corresponded with viruses occurrence in postharvest tubers testing. This methodology is suitable for detection of PVY and PLRV in potato plants, rapid evaluation of aphid infection potential in potato fields and studies of viruses transmissibility by other aphid species.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

<a href="/en/project/QK22010194" target="_blank" >QK22010194: Integrated protection against vectors of viral diseases in seed potatoes and other crops</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2026

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Plant Diseases and Protection

ISSN

1861-3829

e-ISSN

1861-3837

Volume of the periodical

133

Issue of the periodical within the volume

51

Country of publishing house

DE - GERMANY

Number of pages

13

Pages from-to

nestránkováno

UT code for WoS article

001712987600002

EID of the result in the Scopus database

2-s2.0-105033833000