Ms1 RNA increases the amount of RNA polymerase in Mycobacterium smegmatis.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG33__%2F19%3AN0000005" target="_blank" >RIV/60162694:G33__/19:N0000005 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/19:00504361 RIV/68378050:_____/19:00504361 RIV/00216208:11310/19:10393364 RIV/68407700:21340/19:00327594
Result on the web
<a href="https://www.ncbi.nlm.nih.gov/pubmed/30427073" target="_blank" >https://www.ncbi.nlm.nih.gov/pubmed/30427073</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/mmi.14159" target="_blank" >10.1111/mmi.14159</a>

Result language
angličtina
Original language name
Ms1 RNA increases the amount of RNA polymerase in Mycobacterium smegmatis.
Original language description
Ms1 is a sRNA recently found in mycobacteria and several other actinobacterial species. Ms1 interacts with the RNA polymerase (RNAP) core devoid of sigma factors, which differs from 6S RNA that binds to RNAP holoenzymes containing the primary sigma factor. Here we show that Ms1 is the most abundant non-rRNA transcript in stationary phase in Mycobacterium smegmatis. The accumulation of Ms1 stems from its high-level synthesis combined with decreased degradation. We identify the Ms1 promoter, PMs1 , and cis-acting elements important for its activity. Furthermore, we demonstrate that PNPase (an RNase) contributes to the differential accumulation of Ms1 during growth. Then, by comparing the transcriptomes of wt and ΔMs1 strains from stationary phase, we reveal that Ms1 affects the intracellular level of RNAP. The absence of Ms1 results in decreased levels of the mRNAs encoding β and β' subunits of RNAP, which is also reflected at the protein level. Thus, the ΔMs1 strain has a smaller pool of RNAPs available when the transcriptional demand increases. This contributes to the inability of the ΔMs1 strain to rapidly react to environmental changes during outgrowth from stationary phase.
Czech name
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Czech description
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Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
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OECD FORD branch
10606 - Microbiology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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