Rifampicin Nanoformulation Enhances Treatment of Tuberculosis in Zebrafish.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG33__%2F19%3AN0000007" target="_blank" >RIV/60162694:G33__/19:N0000007 - isvavai.cz</a>
Alternative codes found
RIV/61389013:_____/19:00503810 RIV/00216208:11110/19:10394344 RIV/00216208:11130/19:10394344 RIV/00216208:11310/19:10394344 RIV/00064203:_____/19:10394344
Result on the web
<a href="https://pubs.acs.org/doi/10.1021/acs.biomac.9b00214" target="_blank" >https://pubs.acs.org/doi/10.1021/acs.biomac.9b00214</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.biomac.9b00214" target="_blank" >10.1021/acs.biomac.9b00214</a>
Alternative languages
Result language
angličtina
Original language name
Rifampicin Nanoformulation Enhances Treatment of Tuberculosis in Zebrafish.
Original language description
Mycobacterium tuberculosis, the etiologic agent of tuberculosis, is an intracellular pathogen of alveolar macrophages. These cells avidly take up nanoparticles, even without the use of specific targeting ligands, making the use of nanotherapeutics ideal for the treatment of such infections. Methoxy poly(ethylene oxide)- block-poly(ε-caprolactone) nanoparticles of several different polymer blocks' molecular weights and sizes (20-110 nm) were developed and critically compared as carriers for rifampicin, a cornerstone in tuberculosis therapy. The polymeric nanoparticles' uptake, consequent organelle targeting and intracellular degradation were shown to be highly dependent on the nanoparticles' physicochemical properties (the cell uptake half-lives 2.4-21 min, the degradation half-lives 51.6 min-ca. 20 h after the internalization). We show that the nanoparticles are efficiently taken up by macrophages and are able to effectively neutralize the persisting bacilli. Finally, we demonstrate, using a zebrafish model of tuberculosis, that the nanoparticles are well tolerated, have a curative effect, and are significantly more efficient compared to a free form of rifampicin. Hence, these findings demonstrate that this system shows great promise, both in vitro and in vivo, for the treatment of tuberculosis.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10404 - Polymer science
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biomacromolecules
ISSN
1525-7797
e-ISSN
1526-4602
Volume of the periodical
20
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
17
Pages from-to
1798-1815
UT code for WoS article
000464248300033
EID of the result in the Scopus database
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