System with embedded drug release and nanoparticle degradation sensor showing efficient rifampicin delivery into macrophages

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F17%3A00470718" target="_blank" >RIV/61389013:_____/17:00470718 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/17:10360758 RIV/00216208:11130/17:10360758 RIV/00064203:_____/17:10360758
Result on the web
<a href="http://dx.doi.org/10.1016/j.nano.2016.08.031" target="_blank" >http://dx.doi.org/10.1016/j.nano.2016.08.031</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.nano.2016.08.031" target="_blank" >10.1016/j.nano.2016.08.031</a>

Result language
angličtina
Original language name
System with embedded drug release and nanoparticle degradation sensor showing efficient rifampicin delivery into macrophages
Original language description
We have developed a biodegradable, biocompatible system for the delivery of the antituberculotic antibiotic rifampicin with a built-in drug release and nanoparticle degradation fluorescence sensor. Polymer nanoparticles based on poly(ethylene oxide) monomethyl ether-block-poly(epsilon-caprolactone) were noncovalently loaded with rifampicin, a combination that, to best of our knowledge, was not previously described in the literature, which showed significant benefits. The nanoparticles contain a Förster resonance energy transfer (FRET) system that allows real-time assessment of drug release not only in vitro, but also in living macrophages where the mycobacteria typically reside as hard-to-kill intracellular parasites. The fluorophore also enables in situ monitoring of the enzymatic nanoparticle degradation in the macrophages. We show that the nanoparticles are efficiently taken up by macrophages, where they are very quickly associated with the lysosomal compartment. After drug release, the nanoparticles in the cmacrophages are enzymatically degraded, with half-life 88 ± 11 min.
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10404 - Polymer science

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)