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Toward the construction of Francisella tularensis 2-DE database

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG38__%2F00%3A00000302" target="_blank" >RIV/60162694:G38__/00:00000302 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Toward the construction of Francisella tularensis 2-DE database

  • Original language description

    Francisella tularensis is an obligate intracellular pathogen whose genome sequencing will be completed shortly. The next step should be the analysis of functional complement of genetic information so called proteome. Proteom is a new rapidly emerging field of study utilizing the combination of two-dimensional gel electrophoresis (2-DE) and mass spectrometry.We applied 2-DE methodology with immobilized pH gradients in the first dimension for separation of complete protein lysates of attenuated Francisella tularensis strain LVS. Two different techniques 9-16 % gradient SDS-PAGE and Tricine SDS-PAGE were used in the second dimension for the resolution of proteins in the range of molecular weights 10 to 200 kDa and 5-70 kDa, respectively. In the first caseabout 2700 proteins were detected on one gel, regarding Tricine SDS-PAGE we were able to stain approximately 1200 different protein molecules. Besides the cultivation of bacteria under normal condition we also examined the protein expres

  • Czech name

    K vytvoření Francisella tularensis 2-DE datebáze

  • Czech description

    K vytvoření Francisella tularensis 2-DE datebáze

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    EC - Immunology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/LN00A033" target="_blank" >LN00A033: The proteome center for the study of intracellular parasitism of microbes</a><br>

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2000

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    The 3rd International Conference on Tularemia

  • ISBN

    1104-9154

  • ISSN

  • e-ISSN

  • Number of pages

    2

  • Pages from-to

    29-30

  • Publisher name

    FOA Defence Research Establishment

  • Place of publication

    Umea

  • Event location

    Umea, Sweden

  • Event date

    Aug 27, 2000

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article