Construction of a Francisella tularensis two-dimensional electrophoresis protein database

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG16__%2F01%3A00000499" target="_blank" >RIV/60162694:G16__/01:00000499 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/01:53010124 RIV/60162694:G38__/01:00000499

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Construction of a Francisella tularensis two-dimensional electrophoresis protein database

Original language description

We have started the construction of a two dimensional database of the proteome of Francisella tularensis, a bacterium that is responsible for the highly pathogenic disease tularemia. The genome of this intracellular pathogen is not completely sequenced,yet and, currently, information only about 54 proteins is available from NCBI database. We have analyzed the Francisella tularensis live vaccine strain by two-dimensional gel electrophoresis with immobilized pH 3-10 gradient in the first dimension and 9-16 % gradient or tricine SDS-PAGE in the second dimension. In both cases about 2000 spots were detected. Furthermore, we compared the protein pattern of the non-virulent Francisella tularensis live vaccine strain with protein profiles of two wild type clinical isolates and more than 50 differentially expressed proteins were counted. The separated proteins are going to be identified by peptide mass fingerprinting, however, due to the lack of complete genome sequence data only eight protei

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EC - Immunology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2001

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Proteomics

ISSN

1615-9853

e-ISSN

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Volume of the periodical

1

Issue of the periodical within the volume

4

Country of publishing house

DE - GERMANY

Number of pages

8

Pages from-to

508-515

UT code for WoS article

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EID of the result in the Scopus database

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