Deletion of IglH in virulent Francisella tularensis subsp holarctica FSC200 strain results in attenuation and provides protection against the challenge with the parental strain

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F12%3A10133996" target="_blank" >RIV/00216208:11160/12:10133996 - isvavai.cz</a>

Result on the web

<a href="http://www.sciencedirect.com/science/article/pii/S1286457911002309" target="_blank" >http://www.sciencedirect.com/science/article/pii/S1286457911002309</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.micinf.2011.08.017" target="_blank" >10.1016/j.micinf.2011.08.017</a>

Result language

angličtina

Original language name

Deletion of IglH in virulent Francisella tularensis subsp holarctica FSC200 strain results in attenuation and provides protection against the challenge with the parental strain

Original language description

Francisella tularensis, the causative agent of tularemia, is a highly infectious intracellular pathogen with no licensed vaccine available today. The recent search for genome sequences involved in E tularensis virulence mechanisms led to the identification of the 30-kb region defined as a Francisella pathogenicity island (FPI). In our previous iTRAQ study we described the concerted upregulation of some FPI proteins in different E tularensis strains cultivated under stress conditions. Among them we identified the IglH protein whose role in Francisella virulence has not been characterized yet. In this work we deleted the iglH gene in a European clinical isolate of E tularensis subsp. holarctica FSC200. We showed that the iglH gene is necessary for intracellular growth and escape of F. tularensis from phagosomes. We also showed that the iglH mutant is avirulent in a mouse model of infection and persists in the organs for about three weeks after infection. Importantly, mice vaccinated by i

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EC - Immunology

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microbes and Infection

ISSN

1286-4579

e-ISSN

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Volume of the periodical

14

Issue of the periodical within the volume

2

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

11

Pages from-to

177-187

UT code for WoS article

000300819100009

EID of the result in the Scopus database

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