Deletion of IglH in virulent Francisella tularensis subsp. tularensis holarctica FSC200 strain results in attenuation and provides protection against the challenge with the parental strain

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F12%3A00002441" target="_blank" >RIV/60162694:G44__/12:00002441 - isvavai.cz</a>
Result on the web
<a href="http://www.journals.elsevier.com/microbes-and-infection/" target="_blank" >http://www.journals.elsevier.com/microbes-and-infection/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.micinf.2011.08.017" target="_blank" >10.1016/j.micinf.2011.08.017</a>

Result language
angličtina
Original language name
Deletion of IglH in virulent Francisella tularensis subsp. tularensis holarctica FSC200 strain results in attenuation and provides protection against the challenge with the parental strain
Original language description
Francisella tularensis, the causative agent of tularemia, is a highly infectious intracellular pathogen with no licensed vaccine available today. The recent search for genome sequences involved in F. tularensis virulence mechanisms led to the identification of the 30-kb region defined as a Francisella pathogenicity island (FPI). In our previous iTRAQ study we described the concerted upregulation of some FPI proteins in different F. tularensis strains cultivated under stress conditions. Among them we identified the IglH protein whose role in Francisella virulence has not been characterized yet. In this work we deleted the iglH gene in a European clinical isolate of F. tularensis subsp. holarctica FSC200. We showed that the iglH gene is necessary for intracellular growth and escape of F. tularensis from phagosomes. We also showed that the iglH mutant is avirulent in a mouse model of infection and persists in the organs for about three weeks after infection. Importantly, mice vaccinated b
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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