Proteomics analysis of the Francisella tularensis LVS response to iron restriction: induction of the F. tularensis pathogenicity island proteins IglABC

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F07%3A00001875" target="_blank" >RIV/60162694:G44__/07:00001875 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Proteomics analysis of the Francisella tularensis LVS response to iron restriction: induction of the F. tularensis pathogenicity island proteins IglABC

Original language description

Francisella tularensis is a highly virulent, facultative intracellular pathogen that causes tularemia in humans and animals. Although it is one of the most infectious bacterial pathogens, little is known about its virulence mechanisms. In the present study we investigated the response of F. tularensis live vaccine strain (LVS) to iron depletion, which simulates the environment within the host. In order to detect alterations in protein synthesis we used metabolic labeling followed by 2D-PAGE analysis. Globally, we detected 141 protein spots whose levels were significantly altered in the iron restricted medium. About 65% of the spots were successfully identified using mass spectrometric approaches. Importantly, among the proteins produced at increased level during iron-limited growth, we found three proteins encoded by the igl operon, located in the Francisella tularensis pathogenicity island I (FPI). Of these, the IglC and IglA proteins were previously reported to be necessary for full

Czech name

Proteomická analýza odpovědi Francisella tularensis LVS na nedostatek železa: indukce F. tularensis proteinů ostrova patogenity IglABC

Czech description

Prezentovaná práce byla zaměřena na studium vlivu nedostatku zdroje železa na proteom bakterie Francisella tularensis.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/OPUOFVZ200602" target="_blank" >OPUOFVZ200602: Virulence factors of intracellular pathogen Francisella tularensis classified as cathegory A biological warfare agents</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

FEMS Microbiology Letters

ISSN

0378-1097

e-ISSN

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Volume of the periodical

269

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

11

Pages from-to

11-21

UT code for WoS article

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EID of the result in the Scopus database

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