Determination of fumonisins in milled corn grains using HPLC/MS

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F10%3A00002332" target="_blank" >RIV/60162694:G44__/10:00002332 - isvavai.cz</a>

Alternative codes found

RIV/25328859:_____/10:#0000527 RIV/44555601:13440/10:00005559

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Determination of fumonisins in milled corn grains using HPLC/MS

Original language description

A new method for determination of fumonisins in corn samples was developed and validated. The mycotoxins were extracted by a mixture of methanol-acetonitrile-water (1:1:2, v/v/v) and determined on a liquid chromatograph with mass spectrometric detection.The separation was performed on Zorbax XDB-C(18) column (150 x 4.6 mm; 5 microm) with a Metaguard ODS-2 precolumn (30 x 4.6 mm; 5 mum) using gradient elution with mobile phase consisting of acetonitrile and 5 mmol/L ammonium acetate (adjusted by aceticacid to pH 3.0). For detection of (M+H(+)) ions, a quadrupole mass spectrometer in single ion monitoring mode was applied. Developed method showed very good linearity in a tested range of concentration. Detection limit is 62.0 microg FB(1)/kg and 58.5 microg FB(2)/kg of maize grains. Because the detection limits lie under the maximum permitted EU levels, the method is suitable for determination of fumonisins in milled corn grains.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Chromatographic Science

ISSN

0021-9665

e-ISSN

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Volume of the periodical

48

Issue of the periodical within the volume

8

Country of publishing house

US - UNITED STATES

Number of pages

5

Pages from-to

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UT code for WoS article

000281047400012

EID of the result in the Scopus database

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