Spectrophotometric assay of aflatoxin B1 using acetylcholinesterase immobilized on standard microplates
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F13%3A43874952" target="_blank" >RIV/60162694:G44__/13:43874952 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26620/13:PU127101
Result on the web
<a href="http://www.researchgate.net/journal/0172-780X_Neuro_endocrinology_letters" target="_blank" >http://www.researchgate.net/journal/0172-780X_Neuro_endocrinology_letters</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/00032719.2012.757703" target="_blank" >10.1080/00032719.2012.757703</a>
Alternative languages
Result language
angličtina
Original language name
Spectrophotometric assay of aflatoxin B1 using acetylcholinesterase immobilized on standard microplates
Original language description
Aflatoxins are a group of hepatotoxic secondary metabolites produced by molds Aspergillus. During inappropriate storage or processing of food and beverages, aflatoxins can be distributed and become a serious health problem. Disparate analytical techniques were prepared for the assay in the past. Here, a method based on inhibition of enzyme acetylcholinesterase (AChE) is performed allowing fast and simple prove of aflatoxins presence. For the assay purposes, AChE was immobilized on standard polystyrene microplates using gelatin as a stabilizing substance. Both free and immobilized AChEs were used and compared one to each other. Immobilization protocol was optimized and interference of organic solvents such as methanol was tested. For the immobilized AChE, limit of detection 2.75 ppb for aflatoxin B1 was received. The immobilized AChE kept full activity at least one month. Suitability of the assay for a practical performance is considered.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical Letters
ISSN
0003-2719
e-ISSN
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Volume of the periodical
46
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
1306-1315
UT code for WoS article
000318281700011
EID of the result in the Scopus database
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