A novel fluorescence assay and catalytic properties of Crh1 and Crh2 yeast cell wall transglycosylases
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F13%3A43875043" target="_blank" >RIV/60162694:G44__/13:43875043 - isvavai.cz</a>
Result on the web
<a href="http://www.biochemj.org/bj/455/bj4550307.htm" target="_blank" >http://www.biochemj.org/bj/455/bj4550307.htm</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1042/BJ20130354" target="_blank" >10.1042/BJ20130354</a>
Alternative languages
Result language
angličtina
Original language name
A novel fluorescence assay and catalytic properties of Crh1 and Crh2 yeast cell wall transglycosylases
Original language description
The mechanical properties of fungal cell walls are largely determined by composition and mutual cross-linking of their macromolecular components. Previous work showed that the Crh proteins are required for the formation of cross-links between chitin andglucan at the Saccharomyces cerevisiae cell wall. In the present study, the proteins encoded by CRH1 and CRH2 were heterologously expressed in Pichia pastoris and a sensitive fluorescence in vitro soluble assay was devised for determination of their transglycosylating activities. Both proteins act as chitin transglycosylases; they use soluble chitin derivatives, such as carboxymethyl chitin, glycol-chitin and/or N-acetyl chito-oligosaccharides of DP (degree of polymerization) >= 5 as the oligoglycosyl donors, and oligosaccharides derived from chitin, beta-(1,3)-glucan (laminarin) and beta-(1,6)-glucan (pustulan), fluorescently labelled with sulforhodamine or FITC as acceptors. The minimal number of intact hexopyranose units required byC
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
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Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biochemical Journal
ISSN
0264-6021
e-ISSN
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Volume of the periodical
455
Issue of the periodical within the volume
3
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
307-318
UT code for WoS article
000326664100005
EID of the result in the Scopus database
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