Yeast and fungal cell-wall polysaccharides can self-assemble in vitro into an ultrastructure resembling in vivo yeast cell walls

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F13%3A00067534" target="_blank" >RIV/00216224:14110/13:00067534 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1093/jmicro/dfs076" target="_blank" >http://dx.doi.org/10.1093/jmicro/dfs076</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jmicro/dfs076" target="_blank" >10.1093/jmicro/dfs076</a>

Result language

angličtina

Original language name

Yeast and fungal cell-wall polysaccharides can self-assemble in vitro into an ultrastructure resembling in vivo yeast cell walls

Original language description

Polysaccharides account for more than 90% of the content of fungal cell walls, but the mechanism underlying the formation of the architecture of the cell walls, which consist of microfibrils embedded in an amorphous wall matrix, remains unknown. We usedelectron microscopy to investigate ten different fungal cell-wall polysaccharides to determine whether they could self-assemble into the fibrillar or amorphous component of fungal cell walls in a test tube without enzymes. The ultrastructures formed by precipitating beta-1,3-glucan and beta-1,6-glucan are different depending on the existence of branching in the molecule. Linear beta-1,3- glucan and linear beta-1,6-glucan precipitate into a fibrillar ultrastructure. Branched beta-1,6-glucan, mannan and glycogen precipitates are amorphous. Branched beta-1,3-glucan forms a fibrillar plus amorphous ultrastructure.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

—

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microscopy

ISSN

0022-0744

e-ISSN

—

Volume of the periodical

62

Issue of the periodical within the volume

3

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

327-339

UT code for WoS article

000320457100001

EID of the result in the Scopus database

—