Comparison of an Alzheimer disease drug ability to bind acetylcholinesterase using both electrochemical and spectrophotometric assays

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F14%3A43875221" target="_blank" >RIV/60162694:G44__/14:43875221 - isvavai.cz</a>

Result on the web

<a href="http://www.roavs.com/pdf-files/Issue-4-2014/203-207.pdf" target="_blank" >http://www.roavs.com/pdf-files/Issue-4-2014/203-207.pdf</a>

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Comparison of an Alzheimer disease drug ability to bind acetylcholinesterase using both electrochemical and spectrophotometric assays

Original language description

Acetylcholinesterase (AChE) is an enzyme playing important role in termination of neurotransmission within cholinergic system. The enzyme is targeted by many compounds including nerve agents used for chemical warfare, natural toxins and drugs for Alzheimer disease. Thousands of new inhibitors are prepared for pharmaceutical purposes over the year and necessity to characterize them is given for this reason. In this work, standard spectrophotometric test is compared to electrochemical test in order to assay AChE activity and measure its inhibition. The both test were performed for characterization of an Alzheimer disease drug galantamine. The spectrophotometric test was based on standard Ellman's reaction. Electrochemical test used enzymatic hydrolysis to acetylthiocholine with consequent electrochemical oxidation. In the experiments, we proved that electrochemical test was able to reach better limit of detection for galantamine than the standard spectrophotometric test. Calculated media

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

—

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Research Opinions in Animal & Veterinary Sciences

ISSN

2221-1896

e-ISSN

—

Volume of the periodical

4

Issue of the periodical within the volume

4

Country of publishing house

PK - PAKISTAN

Number of pages

5

Pages from-to

203-207

UT code for WoS article

—

EID of the result in the Scopus database

—