Construction of an acetylcholinesterase sensor based on synthesized paramagnetic nanoparticles, a simple tool for neurotoxic compounds assay

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F17%3A43875750" target="_blank" >RIV/60162694:G44__/17:43875750 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43210/17:43911525 RIV/00216275:25310/17:39902675 RIV/00216305:26620/17:PU124060

Result on the web

<a href="http://www.mdpi.com/1424-8220/17/4/676" target="_blank" >http://www.mdpi.com/1424-8220/17/4/676</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/s17040676" target="_blank" >10.3390/s17040676</a>

Result language

angličtina

Original language name

Construction of an acetylcholinesterase sensor based on synthesized paramagnetic nanoparticles, a simple tool for neurotoxic compounds assay

Original language description

Magnetic particles (MPs) have been widely used in biological applications in recent years as a carrier for various molecules. Their big advantage is in repeated use of immobilized molecules including enzymes. Acetylcholinesterase (AChE) is an enzyme playing crucial role in neurotransmission and the enzyme is targeted by various molecules like Alzheimer&apos;s drugs, pesticides and warfare agents. In this work, an electrochemical biosensor having AChE immobilized onto MPs and stabilized through glutaraldehyde (GA) molecule was proposed for assay of the neurotoxic compounds. The prepared nanoparticles were modified by pure AChE and they were used for the measurement anti-Alzheimer&apos;s drug galantamine and carbamate pesticide carbofuran with limit of detection 1.5 mu M and 20 nM, respectively. All measurements were carried out using screen-printed sensor with carbon working, silver reference, and carbon auxiliary electrode. Standard Ellman&apos;s assay was used for validation measurement of both inhibitors. Part of this work was the elimination of reversible inhibitors represented by galantamine from the active site of AChE. For this purpose, we used a lower pH to get the original activity of AChE after inhibition by galantamine. We also observed decarbamylation of the AChE-carbofuran adduct. Influence of organic solvents to AChE as well as repeatability of measurement with MPs with AChE was also established.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30404 - Biomaterials (as related to medical implants, devices, sensors)

Project

<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Sensors

ISSN

1424-8220

e-ISSN

—

Volume of the periodical

17

Issue of the periodical within the volume

4

Country of publishing house

CH - SWITZERLAND

Number of pages

12

Pages from-to

"neuvedeno"

UT code for WoS article

000400822900017

EID of the result in the Scopus database

2-s2.0-85016139336&doi