The impact of pre-analytical treatment and sorting on human neutrophil function

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F14%3A43875327" target="_blank" >RIV/60162694:G44__/14:43875327 - isvavai.cz</a>

Result on the web

<a href="http://www.agriculturejournals.cz/web/vetmed.htm?volume=59&type=volume#No" target="_blank" >http://www.agriculturejournals.cz/web/vetmed.htm?volume=59&type=volume#No</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The impact of pre-analytical treatment and sorting on human neutrophil function

Original language description

This study was designed to test the hypothesis that pre-analytical treatment of relatively fragile cells is more important for their viability and function than shear forces and decompression shocks on a flow cytometric sorter. Human neutrophils were enriched using different techniques ( sedimentation, hypotonic or ammonium chloride-mediated erythrocyte lysis) and their function was evaluated by measuring their capability of mounting oxidative burst. In contrast to other isolation techniques, neutrophils enriched by spontaneous sedimentation were found to be intact both in terms of their function and relative numbers within the leukocyte population; such preparations were thus run on a FACSAria cell sorter using 70, 85 and 100 mu m nozzles and the corresponding internal pressure values. The viability and function of sorted neutrophils with or without subsequent cultivation were re-evaluated using phagocytosis assays. Independent of the nozzle size and internal sorter pressure, the capa

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Veterinární medicína

ISSN

0375-8427

e-ISSN

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Volume of the periodical

59

Issue of the periodical within the volume

11

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

10

Pages from-to

543-552

UT code for WoS article

000349028300004

EID of the result in the Scopus database

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