Isolation techniques of neutrophils and peripheral blood mononuclear cells for the comparative experiments in humans and pigs model organisms in flow cytometry

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F14%3A43908804" target="_blank" >RIV/62156489:43210/14:43908804 - isvavai.cz</a>
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2014/mnet_2014_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2014/mnet_2014_full.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Isolation techniques of neutrophils and peripheral blood mononuclear cells for the comparative experiments in humans and pigs model organisms in flow cytometry
Original language description
The pig is due to the morfology of their bodies often used as a model organism from the experimental organisms for comparison with man. The aim of this study ist o determine which isolation technique can be used for comparative imunological experiments focused on human and porcine neutrophils and monocytes. In comparative studies, it is necessary that both cells compared species were isolated by the same technique. Selected isolation techniques are the first step for subsequent detection of selected parameters in flow cytometry and for cultivation of peripheral blood mononuclear cells to profit monocyte-derivated macrophages. For isolation of neutrophils were chosen these techniques: isolation of double density gradient centrifugation Histopaque 11191a 1077, isolation of double density gradient centrifugation Histopaque 11191 a 1077 after dextran and only dextran sedimentation. Monocytes were isolated by density gradient centrifugation Histopaque 1077, density gradient centrifugation
Czech name
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Czech description
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Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
MendelNet 2014: Proceedings of International Ph.D. Students Conference
ISBN
978-80-7509-174-1
ISSN
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e-ISSN
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Number of pages
6
Pages from-to
516-521
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 19, 2014
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000362518300099

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